Sayedeh Elmira Yazdi-Rouholamini; Nasrin Motamed; Mohammad Tahmasb; Kobra Omidfar
Volume 23, Issue 3 , July and August 2016, , Pages 386-391
Abstract
Background & Objectives: Metastatic cancers, such as breast cancer, are generally resistant to chemotherapy. Therefore many researches try to find new and effective compounds to treat this type of cancer. In this study, we examined the Silibinin toxic effect on tumor growth and proliferation of MCF-7 ...
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Background & Objectives: Metastatic cancers, such as breast cancer, are generally resistant to chemotherapy. Therefore many researches try to find new and effective compounds to treat this type of cancer. In this study, we examined the Silibinin toxic effect on tumor growth and proliferation of MCF-7 cell line. Materials & methods MTT assay was used to assess the inhibitory effect of silibinin on metabolic activity of MCF_7 cells. For this purpose, seeded cells were plated on to 96-well plates and then exposed to varying concentrations of silibinin (0-350 ug/mL) for different time (24, 48 and 72 h). The percentage of metabolic activity was calculated according to the MTT test. In next step, in order to evaluate the effect of silibinin on apoptosis in MCF_7 cells, Caspase 3/7 test was used. Results: Our results indicate that silibinin inhibited metabolic activity of MCF-7cells in a dose-dependent manner and it can induce apoptosis pathway in these cells. Conclusion: The study showed that treatment of MCF-7 cell line with Silibinin can be through induction of apoptosis, inhabitation of the growth and proliferation of cells. Due to the significant inhibitory effect of silibinin on MCF-7 cells, it seems important for researchers to utilize milk thistle in the treatment and prevention of cancer.
Somayyeh Mohammadi; Majid Esmaeelizad; Abdolhamid Angji; Mohammad Tahmasb; Roghayeh Mesri; Mojgan Ahmadzadeh
Volume 22, Issue 1 , March and April 2015, , Pages 66-72
Abstract
Background and purpose: Hydatidosis is a parasitic disease that transmits by Echinococcusgranulosus eggs excreted from the feces of infected dogs to humans and animals. This parasite is one of the tapeworms of the family Taeniidae which causes widespread health problems among humans and also economic ...
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Background and purpose: Hydatidosis is a parasitic disease that transmits by Echinococcusgranulosus eggs excreted from the feces of infected dogs to humans and animals. This parasite is one of the tapeworms of the family Taeniidae which causes widespread health problems among humans and also economic loss in animal. Therefore, designing a new generation vaccine is important to prevent the disease.
Materials and methods: In this study, the linear T-cell epitopes were selected. The coding sequence of epitopes was synthesized and cloned to pEGFP-N1vector. The recombinant vector was transfected into CHO cells by genpulser. Expression of fusion Protein was confirmed by fluorescence microscopy.
Results: The accuracy of cloning was confirmed by both colony PCR on the clone and the subsequent sequencing. Fluorescence microscopy showed fusion protein was expressed in CHO.
Conclusion: The results showed that, linear T-cell epitope of EG95 antigen was cloned correctly in pEGFP vector and its expression confirmed that this recombinant vector can be used to construct a DNA vaccine model against the Echinococcusgranulosus.